KD determination of DB3 and DB3DB3 to monomeric Aβ using biolayer interferometry (BLI).
收藏Figshare2016-06-22 更新2026-04-29 收录
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https://figshare.com/articles/dataset/K_sub_D_sub_determination_of_DB3_and_DB3DB3_to_monomeric_A_using_biolayer_interferometry_BLI_/3196984
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N-terminally biotinylated Aβ(1–42) monomers were immobilized on streptavidin biosensors and the binding of DB3 and DB3DB3 was detected. Representative double referenced sensorgrams of a dilution series of DB3 (A) and DB3DB3 (B) are shown, including the equilibrium dissociation constants (KD) as means ± SD of data recorded in triplicate. For steady state analysis Langmuir´s 1:1 binding model was applied. Representative fits of DB3 (C) and DB3DB3 (D) are depicted with the corresponding corrected R2.
创建时间：
2016-06-22
