The transcriptome analysis of Peri-infarct areas of infarction for sham, PT, and Pushen treatment

We investigated the functional recovery of in ischemic stroke by Photothrombotic stroke (PT) model. RNA-Seq of the peri-infarct cortex at 28 day after PT to clarify the potential mechanisms of pushen capsule against ischemic stroke. Pushen (drug approval number: Z20040074; batch number: 20082403; Suzhong pharma) was dissolved in the vehicle of ultrapure water, and it was administered by oral gavage at 24 hours after surgery continuously for 28 days. Mice in the Sham and Vehicle group were administered with a vehicle of ultrapure water equal to the Pushen group. By applying the cutoffs of P <0.05 and |log2 (fold change)|>1, 2369 differentially expressed genes (DEGs) were identified between sham vs PT, and 231 differentially expressed genes (DEGs) were identified between PT vs Pushen. Peri-infarct areas of infarction from the three groups were respectively collected in TRIzol reagent at 28 days after PT. Each group contained three replicates.