Effect of depletion of C10orf71 on gene expression in hearts from embryonic mice at E18.5 and adult mice

To explore the specific functional and cellular processes that affected by the mC10orf71 deletion, we compared total RNA expression in hearts from E18.5. Gene Ontology (GO) enrichment analysis showed that down-regulated genes were not significantly enriched in any biological process. The 327 genes up-regulated in KO mice were enriched for energy generation, electron transport chain and ATP synthesis, and many of them are encoded by mitochondrial DNA. These changes were likely to be a compensatory response to the impaired morphogenesis and dysfunction of the KO hearts. Then, we performed RNA-seq on hearts of adult WT and KO mice. GO analysis of down-regulated genes identified two categories of biological processes: mRNA processing/splicing and muscle cell differentiation/contraction. We performed gene expression analysis using data obtained from RNA-seq of 4 WT mice and 4 KO mice at two time points为探究mC10orf71基因缺失所影响的具体功能与细胞过程，我们对比了胚胎18.5天（E18.5）小鼠心脏的总RNA表达水平。基因本体（Gene Ontology, GO）富集分析结果显示，下调基因未在任何生物过程中出现显著富集。在敲除（Knockout, KO）小鼠中上调的327个基因，富集于能量生成、电子传递链与ATP合成相关通路，其中多数基因由线粒体DNA编码。上述变化或为敲除小鼠心脏形态发生受损与功能异常的代偿性应答。随后，我们对成年野生型（Wild Type, WT）与敲除型（Knockout, KO）小鼠的心脏开展RNA测序（RNA-seq）实验。对下调基因进行GO分析后，共识别出两类生物过程：mRNA加工/剪接以及肌细胞分化/收缩。本研究基于两个时间点下4只野生型小鼠与4只敲除型小鼠的RNA测序数据，开展了基因表达分析。