Measurement of de novo mRNA synthesis of sterol biosynthesis genes upon viral infection.

Wild Type or Tyk2−/− BMDM were infected with mCMV at an MOI of 1 for 1 h. De novo RNA was labeled between 360 and 390 min post-infection, isolated, and hybridized to Affymetrix Gene 1.0 ST microarrays (Materials and Methods). After scanning and data capture, gene expression in mock-infected or infected cells was analyzed, and for the purposes of presentation, gene expression values from control (mock infected) BMDM (black) were adjusted to a value of 1. Values for expression in infected cells (white) were then expressed as a number relative to the control.