Kras G12D Mutant vs. Wildtype

The experiment is a study of the effects of signal strength in the Ras pathway. In particular, we studied a gain-of-function mutant of Kras, KrasG12D. We generated these mutant mice and performed microarray analyses on RNA extracted from whole skin, comparing KrasG12D mice to wild-type mice, with three replicates of each.本实验旨在研究Ras通路（Ras pathway）中信号强度的作用效应。具体而言，我们针对Kras的功能获得性突变体KrasG12D展开研究。我们构建了该突变小鼠模型，并对从全皮肤组织中提取的RNA开展基因芯片（microarray）分析，将KrasG12D突变小鼠与野生型小鼠进行对照，每组设置三次生物学重复。