B-cell epitope prediction using second generation methods.

aAccuracy measures the percentage of correct epitope classification across all residues.bAroc is the area under the curve constructed by the Receiver Operational Characteristics (ROC), which is the function of the sensitivity and specificity of the epitope mapping score. Aroc = 1 indicates perfect prediction of epitopes, Aroc = 0.5 indicates completely random predictions.cp-value is the probability that the observed Aroc value was obtained by chance. In the null positive and null negative methods, all and no residues, respectively, were classified as epitopes.Aroc standard errors (SE) were estimated using bootstrapping. p-values were calculated using permutation tests [6].