ChIP-seq with H3K27Ac antibody

Chromatin immunoprecipitation assay was conducted in rat SNc in sham and model groups. In brief, rat SNc in sham and model groups was gently homogenized to form a single-cell suspension. The suspension was cross-linked for 10 min. Then, after cell lysis, we collected the nuclei of samples. DNA was digested by micrococcal nuclease for 20 min, and the reaction stopped by 0.5 M EDTA. Subsequently, we collected the Nuclear pellet and then incubation in ChIP buffer with protease inhibitors conducted for 10 min on ice. After sonication, the sheared DNA-protein complexes were collected and then were immunoprecipitated with either H3K27Ac or IgG antibody on a rotator for 18 h. Protein G magnetic beads captured DNA-protein complexes. Following capture, DNA fragments were eluted and extracted. The raw reads of samples for rn6 species using ChIP-seq technology service provided by BGI-tech.