Replication Data for: Embryos and tadpoles of the eurythermal Baja California chorus frog (Pseudacris hypochondriaca) show subtle phenotypic changes in response to daily cycling temperatures

This dataset contains data to accompany the publication: Mueller CA, Leão CCB, Atherley KR, Campos N, Eme J. Embryos and tadpoles of the eurythermal Baja California chorus frog (Pseudacris hypochondriaca) show subtle phenotypic changes in response to daily cycling temperatures. Ecol. Evol. Physiol. Accepted Manuscript.<br>
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The files include Excel files with hatch and tadpole data, a R code file for all analyses performed, and tadpole swim videos. <br>
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Abstract of study:
Environmental thermal fluctuations influence fitness-related organismal traits. Investigations of ectothermic physiology must include cycling thermal regimes because such fluctuations are increasing with environmental change. We used the eurythermal Baja California chorus frog (Pseudacris hypochondriaca) to examine developmental responses to daily temperature cycles informed by recorded field conditions (15°C Constant, cycles of 12.5⟷17.5°C and 10⟷20°C) and an extreme daily cycle (15⟷25°C). We measured survival, development rate, mass, and oxygen consumption rate (V̇O2) upon hatch. The 15⟷25°C daily cycle decreased time to hatch, produced larger hatchlings, and reduced mass-specific V̇O2. Lower mass-specific V̇O2 in 12.5⟷17.5°C hatchlings indicated a minor effect of daily temperature cycles with a common mean (15°C). We also measured size, thermal tolerance, V̇O2, and swim speed at a common tadpole stage (hindlimb toe differentiation), with V̇O2 and swim speed measured at 10, 15, 20 and 25°C. 15⟷25°C tadpoles were smaller, but showed higher thermal tolerance, higher mass-specific V̇O2 at 25°C, and higher length-corrected sustained swim speed compared to 15°C Constant and 12.5⟷17.5°C tadpoles. The 15⟷25°C treatment with a higher mean (~20°C) drove most phenotypic differences in hatchlings and tadpoles. Significantly higher thermal tolerance and moderate, but statistically indistinguishable increases in swim speedfor the 10⟷20°C treatment compared to 15°C Constant illustrate subtle effects of daily temperature cycles on tadpole phenotypes. Developing chorus frogs function well at and above maximum temperatures experienced naturally, and our results indicate a subtle ‘warmer is better’ acclimation response for physiological traits in response to substantial daily thermal cycles.