Gene expression profile of alveolar macrophages (AM) from bleomycin treated mice.

Osteopontin (OPN, Spp1-/-) is considered detrimental in pulmonary fibrosis. However, effect of OPN produced by AMs during pulmonary fibrosis is not known. We used bulk RNA sequencing to analyze the effect of OPN in AMs from bleomycin treated mice. Overall design: Alveolar macrophages of wild type (WT) and osteopontin knock-out (Spp1-/-) mice were isolated by Fluorescence-activated cell sorting (FACS) according to the cell surface markers and analyzed using scRNAseq.