Characterization of the Lp-MVs by flow cytometry

Characterization of purified extracellular vesilcles from shed from Legionella pneumophila by flow cytometry
Conclusion:
Due to small size of the vesicles, with diameters smaller than the wavelength of the lasers, it makes it quite challenging to analyze and to distinguish them from the background noise and therefore labeling of the vesicles is mandatory. For standardization, we used the Megamix-Plus SSC (BioCytex) beads. However, synthetic beads have a very different refractive index than MVs, thus makes the scale calibration by FC most inaccurate. MACSQuant performance was calibrated daily using CS&T Research Beads (BD Biosciences). Optics, Fluidics and Gating was adjusted dependent on the output of the Megamix-Plus SSC standardization beads according to the application recommendations by BioCytex.