Influence of oleic acid on rumen fermentation and fatty acid formation in vitro.doc

A series of batch cultures were conducted to investigate the effects of oleic acid (OA) on <i>in vitro</i> ruminal dry matter degradability (IVDMD), gas production, methane (CH₄) and hydrogen (H₂) production, and proportion of fatty acids. Rumen fluid was collected from fistulated goats, diluted with incubation buffer, and then incubated with 500 mg <i>Leymus chinensis</i> meal supplemented with different amounts of OA (0, 20, 40, and 60 mg for the CON, OA20, OA40 and OA60 groups, respectively). Incubation was carried out anaerobically at 39℃ for 48 h, and the samples were taken at 12, 24 and 48 h and subjected to laboratory analysis.