RNAseq analysis of FAP cell states isolated from mdx mice and cultured in vitro. RNAseq analysis of FAP cell states isolated from mdx mice and cultured in vitro

We investigated the transcriptome of cell states identified in fibro/adipogenic progenitors (FAPs) isolated from mdx mice using as a criterion SCA-1 expression. We found that after 5 days in growth medium FAP cell states have different transcriptomes. In particular, among the "Biological process" GO terms enriched in genes up-regulated in SCA1-High-FAPs we found genes correlated with adipogenic differentiation. Indeed, several genes directly regulated by PPAR-gamma are up-regulated in SCA1-High-FAPs. This experiment demonstrates that the adipogenic propensity of SCA1-High-FAPs arise spontaneously in vitro. Overall design: The experiment was performed in four biological replicates (FAP cell states, called SCA1-High-FAPs and SCA1-Low-FAPs, isolated from 4 mdx mice for a total of 8 samples). FAP cell states were cultured 5 days in growth medium ad next we isolated total RNA. SCA1-Low-FAPs were used as a control.