CircRNA as Predictive Biomarker of Penumbra in Acute Ischemic Stroke

Acute ischemic stroke (AIS) is a leading cause of disability and mortality worldwide. By high-throughput sequencing of infarct and ischemic penumbra tissue from middle cerebral artery embolization (MCAO) mice, we identified the CircRNA expression was dramatically and selectively regulated in the penumbra tissues. Overall design: The permanent middle cerebral artery occlusion mice were established by intraluminal filament 3 h after MCAO. The RNA sequencing analysis was performed on the infarction core (core close to frontal lobe and occipital lobe, respectively) and penumbra cortex tissues obtained from six 3h-MCAO mice. The RNA extracted from tissues of six mice was mixed before library construction, separately.