Ultrasonic vocalisation sequence emitted during vocalised urination by a female C57BL/6J mouse
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We recorded undisturbed dyadic interactions between two familiar female mice of the same age (8-12 weeks of age) for 47h (two days and nights). Ultrasonic vocalisations were recorded with one CM16/CMPA microphone (Avisoft Bioacoustics, Glienecke, Germany) connected to the Avisoft Ultrasound Gate 416 (300 kHz sampling rate, 16-bit format; trigger: level of this channel; pre-trigger: 1 s; hold time: 1 s; duration > 0.005 s; trigger event: 2 % energy in 25-125 kHz with entropy < 50%; Avisoft Bioacoustics, Glienecke, Germany). The USV sequence was recorded when one mouse was urinating in a corner of the cage. The given stereo files provide on one channel two repetitions of the USV sequence separated by 2 s of silence, after a high-pass filter at 30 kHz. The second channel includes a repetition of two sequences of background noise separated by 2 s of silence, after a high-pass filter at 30 kHz.



