Figure S1 - Two Lysines in the Forkhead Domain of Foxp3 Are Key to T Regulatory Cell Function
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K17R and K18R did not inhibit the activity of NFAT1 on NFAT∶AP-1 site. 293T cells were transfected with NFAP1-luciferase vector, NFAT1 and WT Foxp3 or K17R or K18R. Forty hours later, cells were stimulated with 1 mM ionomycin, 10 nM PMA, and 2 mM CaCl2 for 6 hours, dual luciferase activity was measured and results normalized to renilla. (PPT)
创建时间：
2012-01-11
