Effect of overexpression or knockdown of trophoblast-related gene GPRC5A on migration and invasion of esophageal squamous cell carcinoma

Emerging evidence suggests that cancer cells possess the capacity to disseminate at early stages, preceding the development of conventional macro-metastases. Nevertheless, the mechanisms governing the seeding and transition of these early disseminated cancer cells (DCCs) into metastatic tumors remain largely unknown. Strikingly, we showed that GPRC5A-overexpressing ESCC cells exhibited an enhanced capacity to effectively implant, survive, and persist, ultimately giving rise to macro-metastases in the lungs in vivo. Therefore, we try to find the potential mechanism of GPRC5A-involved tumor implantation mechanism via overexpressing or silencing GPRC5A in vitro. Overall design: We established GPRC5A-overexpressing/Control KYSE30 cells and GPRC5A-Shcontrol/GPRC5A knockdown KYSE520 cells.