Inducible costimulator ligand (ICOSL) on CD19+ B cells is involved in immunopathological damage of rheumatoid arthritis (RA)

To explore the potential function of CD19+ICOSL- B-cell-subset, the whole transcriptome of sorted CD19+ICOSL+ and CD19+ICOSL- B cells was profiled by using Microarray analysis. CD19+ICOSL+ B cell and CD19+ICOSL- B cell subsets were sorted by FACSAriaTM Ⅱ Cell Sorter (BD Biosciences). Microarray analysis of sorted CD19+ICOSL+ B and CD19+ICOSL- B cells were carried out by Capitalbio Technology Corporation (Changping District, Beijing, China).