Introgression of a synthetic sex ratio distortion transgene into different genetic backgrounds of Anopheles coluzzii

In order to demonstrate the phenotypic stability of a sex ratio distorter in distinct genetic backgrounds of Anopheles mosquitoes, we introgressed a sex-ratio transgene from the donor line Ag(PMB)1 by six serial backcrosses into two recipient colonies of An. coluzzii that had been isolated in Mali (ML) and Burkina Faso (BF). Life history parameters (number of eggs, hatching rate, larval mortality, pupal mortality and sex ratio), adult survival in small cages, 2La inversion karyotyping data (PCR Assay) and species-specific SNP genotyping dataset (KASP Assay) recorded in the donor parental strain Ag(PMB)1, two wild-type An.coluzzii recipient colonies, ML and BF at generation 1, 2, and 7, transgenic F1 and BC1 generations produced during introgression processes, and two backcrossed transgenic BC6 progenies were provided in five distinct excel files as raw data.