The gene expression profiles of maturing osteoclasts (OCs) co-cultured with prostate cancer (PCa) cell lines.

Bone metastatic lesions of PCa contain areas of bone destruction and formation that are directed by OCs and osteoblasts (OBs), respectively. Bone resorption induced by OCs is a necessary priming event for tumor progression in PCa bone metastasis.; however, the characteristics of OCs affected by PCa have not been fully elucidated. Here, we performed NGS analysis to investigate the characteristics of OCs in the presence of PCa. Overall design: To investigate the impact of secretome derived from PCa cells on the molecular characteristics of OCs, we cultured murine monocytic cell line RAW 264.7, which has potential to differentiate into OC-like cells, with metastatic PCa cell lines and controls. We prepared different types of OCs ; OCs normally differentiated from RAW264.7 cells (OC cells), OC cells co-cultured with normal prostate epithelial cells: PNT2 cells (OCN cells), osteolytic PCa cells: PC3M cells (OCP cells), and osteoblastic PCa cells; C4-2B cells (OCC cells). OC differentiation was induced in the presence of RANKL.