Genome integrity maintaining variants related to ionizing radiation resistance in the Lithuanian Chornobyl clean-up workers cohort_Set of the six sub-groups of genes

Study sample consisted of 40 male LCCWs. Two individuals with a history of cancer were removed from our research, ending up with a total of 38 individuals. Data was collected roughly 34 years after the Chornobyl nuclear disaster (in 2019-2020). The age variation of LCWCs was between 50–78 years. Whole-genome sequencing (WGS) and quality control were performed using the Illumina NovaSeq 6000 (Illumina, San Diego, CA, USA) sequencing system and standard DRAGEN version 3.6.4 workflow (the Centre for Genomics and Transcriptomics (CeGaT), Germany). Sequencing was performed at coverage of 26.88–61.38× (an average of 36.27×) per both sample groups. Raw sequencing data (.fastq files) quality was analyzed with FastQC (version 0.11.5-cegat), Q30 values were above 88.59%. Gene-set variation data was extracted by filtering out DNA sequences of the LCCWs according to the particular genomic positions. For each of the analyzed genetic variants quality control steps were: Hardy–Weinberg equilibrium (HWE) (0.001), minor allele frequency (MAF) (0.01), and duplicated variants removal using PLINK v1.9 software (Purcell et al., 2007).