Liquid Chromatography-Mass Spectrometry Method Validation for β-Hydroxythiofentanyl

This dataset corresponds to the method validation process of a Liquid Chromatography-Mass Spectrometry (LC-MS) analytical approach developed for the detection and quantification of β-Hydroxythiofentanyl—a synthetic opioid with high pharmacological activity and potential forensic/toxicological relevance. The dataset encompasses key validation parameters, including linearity (calibration curve data across relevant concentration ranges), accuracy (recovery rates from matrix-matched samples such as plasma, urine, or tissue homogenates), precision (intra-day and inter-day repeatability with relative standard deviations), selectivity (peak purity data and interference testing against structurally similar opioids), limit of detection (LOD) and limit of quantification (LOQ), matrix effect (ion suppression/enhancement assessment), and stability (data on short-term, long-term, freeze-thaw, and post-preparation stability of β-Hydroxythiofentanyl in matrices). Additionally, the dataset includes critical experimental details: LC conditions (column type, mobile phase composition, flow rate, injection volume), MS parameters (ionization mode, collision energy), sample preparation protocols (extraction method, cleanup steps), and replicate measurement data for quality control (QC) samples. This dataset serves as a foundational resource for researchers in forensic toxicology, clinical chemistry, or analytical chemistry seeking to adopt, verify, or optimize LC-MS methods for β-Hydroxythiofentanyl analysis, and supports reproducibility in studies involving this synthetic opioid.