Supporting data for "Comparative maternal protein profiling of mouse biparental and uniparental embryos"

Maternal proteins have important roles during early embryonic development. However, our understanding of maternal proteins is still very limited. The integrated analysis of mouse uniparental (parthenogenetic) and biparental (fertilised) embryos at the protein level creates a protein expression landscape that can be used to explore pre-implantation mouse development. Using label-free quantitative mass spectrometry (MS) analysis, we report on the maternal proteome of mouse parthenogenetic embryos at pronucleus, 2-cell, 4-cell, 8-cell, morula and blastocyst stages, and highlight dynamic changes in protein expression. In addition, comparison of proteomic profiles of parthenogenotes and fertilised embryos highlight the different fates of maternal proteins. Enrichment analysis uncovered a set of maternal proteins that are strongly correlated with the subcortical maternal complex (SCMC), and we report that in parthenogenotes some of these maternal proteins escape the fate of protein degradation. Moreover, we identified a new maternal factor - Fbxw24 - and highlight its importance in early embryonic development. We report that Fbxw24 interacts with Ddb1-Cul4b, and may regulate maternal protein degradation in mouse. Our study provides an invaluable resource for mechanistic analaysis of maternal proteins, and highlights the role of the novel maternal factor Fbw24 in regulating maternal protein degradation during pre-implantation embryo development.