Expression analysis of mice hepatic NMuLi cells treated LNA gapmer ASO containing nucleobase modification

Antisense oligonucleotide (ASO) has the potential to induce hybridization-dependent effects by inadvertent binding of ASOs to RNA with sequences similar to that of the target RNA. In the present study, we examined the effects of the nucleobase derivatives introduced into the gapmer ASOs on gene expression. We performed microarray analysis using NMuLi cells (mouse liver-derived cells) treated with LNA gapmer ASO containing nucleobase modification. The NMuLi cells were seeded in 12-well plates (n = 4/group) and transfected with the LNA-gapmer ASOs using Lipofectamine® 2000 according to the manufacturer’s protocol.Cells treated with transfection reagent in the absence of ASO were used as a control. After a further 24 h, total RNA was isolated.