ORAI1 is required for T cell mediated allergic airway inflammation but not antiviral immunity in the lung

This study investigates the role of store-operated calcium entry (SOCE) in T cell-mediated immune responses to pulmonary influenza A virus (IAV) infection and allergic airway inflammation after immunization by house dust mite (HDM) allergens. We conducted a comparative gene expression analysis of antigen-specific CD4+ T cells from wildtype (WT) and Orai1fl/fl Cd4Cre mice that were adoptively transferred to TCRalpha knockout mice followed by sensitization / challenge with HDM or infection with influenza A virus (IAV) strain x31. Donor CD4+ T cells were isolated from the lungs of host mice at days 9 (IAV) and 14 (HDM), RNA was isolated and processed for bulk RNA sequencing. Overall design: Comparative transcriptome analysis of wildtype (WT) and ORAI1-deficient CD4+ T cells isolated from the lungs of mice after influenza A virus (IAV) infection or house dust mice (HDM, asthma) challenge