MagnetoGUI

This Matlab GUI script is used to analyzed tif stack files. Run Save MagnetoGUI.m and MagnetoGUI.fig in the same folder before running MagnetoGUI.m in Matlab.<br>Instructions for Use:1. Click Load mRuby and select mRuby file2. Adjust threshold and disk size (if appropriate). Click enter to setnew value3. Click Detect Cells4. Inspect image to assure that all cells are being detected. Adjustparameters if necessary and click Detect cells again. Repeat until allcells are delected5. Click Load Video6. Select Fluo4 or ArcLight and THEN adjust fps, StimON, StimDUR,PeakH, PeakW as necessary. Click enter to set new value. This stepcan really be done anytime before step 7. Don't mess with these whilethe analysis is running though; it'll confuse it.7. Click Detect Cells (the one below Load Video).1. This detects all the cells in the field of view. It's used togenerate transfection stats; if you're not going to save the data,you don't need to worry about this step. Just hit Detect Cells so theAnalyze button is enabled. It'll run a little faster if you set thethreshold higher because it won't detect any cells :)7. Click Analyze8. If plots are acceptable, click Save or adjust parameters and clickAnalyze again1. Clicking Saves the mRuby + cell detection, first video frame +cell detection, panel 2 and enclosed plots, the raster plot, anddata sufficient to rerun analysis in a new folder in the samefolder the data analyzed was found2. Checking Categorized Save sorts the data into the appropriatefolder based on the construct and stimulation type. It also savesthe integral data and transfection stats in the appropriate foldersfor creating the heat map/3D bar plot and transfection stats table9. If you would like to reload data, make sure it has been analyzed bythis GUI before (to ensure that the necessary parameters have beenproperly saved) and click Load Old Data to display the analysis10. Data can be reanalyzed by adjusting the parameters and clickinganalyze<br>