Validation of select screen hits by lentiviral infection and flow cytometry.

Up to 2 guides per gene were used, in up to 3 replicates. Each dated column represents the average log2 tagBFP fluorescence for cells infected with that guide, normalized to the average of 2 nontargeting guides (NT). Blank cells mean that no infection for that guide was performed on that day. For each gene, the average of all days and guides is computed, as well as the t-test p value compared to the nontargeting guides, the rank of that gene by p-value, the Benjamini-Hochberg corrected p-value, and the -log of that value, which was used for plotting S2B Fig. (TSV)