Data from: Effects of fluorescent glutamate indicators on neurotransmitter diffusion and uptake
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https://datadryad.org/dataset/doi:10.5061/dryad.573n5tb4q
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资源简介：
Genetically encoded fluorescent glutamate indicators (iGluSnFRs) enable
neurotransmitter release and diffusion to be visualized in intact tissue.
Synaptic iGluSnFR signal time courses vary widely depending on
experimental conditions, often lasting 10-100 times longer than the
extracellular lifetime of synaptically released glutamate estimated with
uptake measurements. iGluSnFR signals typically also decay much more
slowly than the unbinding kinetics of the indicator. To resolve these
discrepancies, here we have modeled synaptic glutamate diffusion, uptake
and iGluSnFR activation to identify factors influencing iGluSnFR signal
waveforms. Simulations suggested that iGluSnFR competes with transporters
to bind synaptically released glutamate, delaying glutamate uptake.
Accordingly, synaptic transporter currents recorded from
iGluSnFR-expressing astrocytes in mouse cortex were slower than those in
control astrocytes. Simulations also suggested that iGluSnFR reduces free
glutamate levels in extrasynaptic spaces, likely limiting extrasynaptic
receptor activation. iGluSnFR and lower-affinity variants nonetheless
provide linear indications of vesicle release, underscoring their value
for optical quantal analysis.
提供机构：
Dryad
创建时间：
2020-07-15
