PIWI-associated RNAs (piRNAs) support germline by suppressing retrotransposons and genes. In mice, piRNAs are essential for spermatogenesis but not oogenesis. To test how this applies to other mammals
Sequencing analyses of total mRNAs in delta-mbt-OSC and OSC (Control). Sequencing analyses of total small RNA, and Piwi-bound, Aub-bound, AGO3-bound piRNAs in delta-mbt-OSCs.
PIWI-clade Argonaute proteins silence transposon expression in animal gonads. Their target specificity is defined by bound ~23-30nt piRNAs that are processed from single-stranded precursor transcripts
This study examines the conservation of Piwi-interacting RNA (piRNA) clusters that come from protein coding gene transcripts. By sequencing small RNA libraries from gonad tissues of Drosophilids and