Comparative Transcriptome Analysis Providing Resistance Mechanism of <i>Aspergillus oryzae</i> Under Arsenate Stress

The molecular resistance mechanism of <i>Aspergillus oryzae</i> (<i>A. oryzae</i>) under arsenate (As(V)) stress was investigated by comparative transcriptome analysis. In total, 4911 differentially expressed genes (DEGs) of <i>A. oryzae</i> were detected under As(V) stress, including 2103 up-regulated genes and 2808 down-regulated genes. Genes induced by As(V) were involved in metal ion binding and transportation, redox homeostasis, efflux carrier, DNA repair, volatilization, etc. Moreover, eight genes were selected for qRT-PCR verification, and their trends were consistent with RNA-Seq analysis, which proved that RNA-Seq analysis was efficient and reliable. The results indicated that the extracellular and intracellular synergistic resistance mechanisms contributed to the improvement of <i>A. oryzae</i> resistance to As (V).