Small RNA sequencing of the 8 to 30-nucleotide window in homo sapiens

RNA-sequencing (RNA-Seq) protocols and bioinformatic pipelines are designed to streamline downstream analyses on sequences believed to be the most important. Here, we have challenged this dogma by preserving ribosomal RNA (rRNA) in our samples and by lowering the minimal RNA size window of our small RNA-Seq analyses to 8 nt Analyzing the content of the RNA size window between 8 and 30 nt of 2 different biological samples from Homo sapiens by small RNA-Seq.