The barrier to radial oxygen loss protects roots against hydrogen sulphide intrusion and its toxic effec

The root barrier to radial O2 loss (ROL) is a key root trait preventing O2 loss from roots to anoxic soils thereby enabling root growth into anoxic, flooded soils. It was well known that the formatuon of ROL barrier was induced in response to the tagnant, deoxygenized treatments. Importantly, We founded that low H2S concentrations induced the formation of a ROL barrier.Since the ROL barrier was formed in response to both H2S and stagnant, deoxygenized treatments, a shared gene network between the two conditions likely exists. Therefore, we performed RNA-seq analysis on tissues from the outer part of the root using laser microdissection on roots grown in the aerated nutrient solution without or with H2S, or in stagnant solution.