Detection of continuous hierarchical heterogeneity by single-cell surface antigen analysis in the prognosis evaluation of acute myeloid leukaemia

In this study, we provided a CyTOF analysis pipeline to find out the AML heterogeneity dynamics along the pseudotime. Minute differences in temporal traits among samples could be exhibited on a trajectory, thus providing a new strategy for predicting AML relapse and monitoring drug responses over time scale. Here are mass cytometry datasets without pre-processing.