On- and off-target mutations in viral genomes induced by Cas9:gRNA-175 in the ADV-EGFP progeny virus.

OTC indicates an off-target candidate (with the number of sites as shown in Supplementary Tables S3, S4). Mismatches in the target sequence (20-nt gRNA175 hybrid region and 3-nt PAM sequence) are bolded and underlined. The mean indel mutation frequencies were determined using deep sequencing (N = 3). N.D., none detected. The sequence locus indicates the position on ADV-EGFP genome. (−), negative strand; (+), positive strand.