慢传输型便秘宏基因组学研究 （Slow transit constipation metagenomics research）

Clostridium difficile and Crohn’s disease are two chronic, often relapsing, immunologically mediated gastrointestinal disorders. Since fecal microbiota transplantation (FMT) has been proved to be efficient in treating Clostridium difficile infection disease, recently it is also being applied to other diseases such as Inflammatory bowel disease whose main forms are Crohn’s Disease (CD) and Ulcerative Colitis (UC). While the efficacy and safety of FMT for UC patients have been assessed, only a few case series were reported on CD patients. To describe and quantify the change of gut flora of IBD patients especially CD patients after FMT, we monitored strain populations in 44 fecal samples. We observed the transfer of donor strains to recipient was more abundant in UC than in CD patients, persisting the follow-up time points. Besides, same-donor recipient differs in the degree of microbiota transfer. Furthermore, we also tended to uncover the principles of microbiota engraftment via random forest classification and regression model. Results showed that both the presence and abundance of some post-FMT patients’ species were predicable, indicating a possibility of precision engineering of the recipients’ gut microbiota under the FMT treatment.