five

pig gut metagenome Metagenome

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NIAID Data Ecosystem2026-03-13 收录
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https://www.ncbi.nlm.nih.gov/sra/SRP377602
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We used plant-diet derived digesta inocula prepared from jejunal and colonic digesta of weaned piglets to study the mucosal response pre- and postweaning towards the microbial and metabolite composition in the inocula. In order to do so the jejunal and colonic inocula were perfused into intestinal loops in a so called intestinal loop perfusion assay. To deeply characterize the bacterial community in the jejunal and colonic inocula, DNA and RNA was extracted and samples were processed using shotgun metagenomic and metatranscriptomic analysis on Illumina HiSeq. was performed as described in Quijada et al. (2022). FASTQ files were obtained after conversion of the BAM files using BedTools v2.29.2 and Samtools 1.7
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2022-07-31
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