A Balance Between Intermediate Filaments and Microtubules Maintains Nuclear Architecture in the Cardiomyocyte

In this manuscript we reported a unique role for desmin in the maintence of nuclear morphology. We performed this LaminB chip-seq to determine if there was a change in LAD organization with desmin KD. LaminB chip-seq was performed from n=3 biological replicates of scrambled (control) or shDesmin treated cells. Each ChIP was paired with an input, and after sequencing, all three replicates were unionized. After input normalization, BigWig files were generated from the control and shDesmin conditions. A bed file of LADs in the control sample is included; no LADs were defined in the shDesmin condition. Some libraries were re-run as noted below.