Chromatin remodeling enzyme Brg1 is required for mouse lens fiber cell terminal differentiation and their denucleation [eyeball tissue]

Genome-wide approach to identify the cell-autonomous role of Brg1 in lens fiber cell terminal differentiation. Differential gene expression was analyzed in Brg1 lens-conditional knockout and wildtype newborn mouse eyeballs, with subsequent comparison of this data with the dnBrg1 mouse lenses expression data. Keywords: Differential gene expression Three biological replicate experiments were performed.本研究采用全基因组水平分析策略，旨在明确Brg1在晶状体纤维细胞终末分化中的细胞自主性功能。对Brg1晶状体条件性敲除型与野生型新生小鼠眼球开展差异基因表达分析，并将所得数据与dnBrg1小鼠晶状体表达谱数据进行比对。关键词：差异基因表达。共完成三次生物学重复实验。