Tissue-naive multimodal profiling of circulating tumor DNA in head and neck squamous cell carcinoma

Circulating tumor DNA (ctDNA) detection at low fractional abundance within plasma cell-free DNA can be challenging without confirmation of shared molecular features within the tumor. We evaluated the feasibility of tumor-naive multimodal molecular profiling of cell-free DNA by interrogating orthogonal genetic and epigenetic features reflective of ctDNA biology.