Transcriptional profiling of Primary Human Hepatocytes (PHH) depleted for PXR and treated with CITCO or Phenobarbital in -/+EGF medium

In primary human hepatocytes (PHH) the involvement of the Pregnane X Receptor (PXR) in genes regulations by Phenobarbital (PB) has never been evaluated at the transcriptomic level. Here we investigated the impact of PXR depletion and epidermal growth factor on PXR dependent gene modulation by PB in PHH. The potential crosstalk with CAR was investigated using Phenobarbital and the direct CAR activator (CITCO) in presence or in absence of EGF. Primary Human Hepatocytes were isolated from 5 different human donors. Cells were cultured in presence or in absence of EGF for 6 days. Transfections with siRNA unspecific (siCT) or targeting PXR (siPXR) were performed at day 2 and 5. RNA was extracted at D6 after 24h treatment with DMSO, CITCO (1µM) or PB (0.5mM). Transcriptomic profiling was performed using Affymetrix HG-U133+PM arrays.