RNAseq and ribosome profiling of yeast cells grown under glucose restriction condition.

We investigate the mechanism by which glucose restriction extends yeast replicative lifespan, using an approach that combines ribosomal profiling and RNA-seq. We systematically compared the translational and transcriptional profiles of cells grown in glucose restriction and normal media, uncovering groups of functionally related genes that are up or down regulated.本研究结合核糖体谱（ribosomal profiling）与RNA测序（RNA-seq）技术，探究葡萄糖限制（glucose restriction）延长酵母复制型寿命（yeast replicative lifespan）的分子机制。本研究系统比较了葡萄糖限制与正常培养基中培养的酵母细胞的翻译组与转录组特征，揭示了功能相关的上调及下调基因类群。