NGS data related to Rajaram et al.: Development of super-specific epigenome e...
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Method overview To achieve targeted ASM, transient transfection of the dCas9-10X SunTag-BFP, scFv-DNMT3A-3L-sfGFP, and sgRNA-DsRed plasmids was performed in HEK293 cells....
方法概览 为实现靶向等位基因特异性甲基化（Allele-Specific Methylation, ASM），本研究在人胚肾293（Human Embryonic Kidney 293, HEK293）细胞中对失活CRISPR相关蛋白9（dead CRISPR-associated protein 9, dCas9）-10X SunTag-蓝色荧光蛋白（Blue Fluorescent Protein, BFP）、单链可变片段（single-chain variable fragment, scFv）-DNA甲基转移酶3A-3L（DNMT3A-3L）-超折叠绿色荧光蛋白（superfolder GFP, sfGFP）及单引导RNA（single guide RNA, sgRNA）-DsRed质粒实施了瞬时转染……
