SPI1 (PU.1), CEBPA and DEK bind the promoter of the CSF3R (G-CSFR) gene

The promoter of the CSF3R gene contains 2 binding sites for SPI1 (PU.1) in the 5' untranslated region (Smith et al. 1996). The 3' site binds SPI1 less strongly (Smith et al. 1996). SPI1 and CEBPA appear to act synergistically in activating transcription of CSFR3. Chromatin immunoprecipitation indicates CEBPA and DEK1 together bind the CSF3R promoter and depletion of DEK1 reduces activation of transcription by CEBPA (Koleva et al. 2012).

CSF3R基因的启动子区域在5'非翻译区含有两个SPI1（PU.1）的结合位点（Smith等人，1996年）。其中，3'位点的结合力相对较弱（Smith等人，1996年）。SPI1与CEBPA似乎在激活CSFR3转录方面协同作用。染色质免疫沉淀实验表明，CEBPA和DEK1共同结合于CSF3R启动子，而DEK1的耗竭会降低CEBPA激活转录的能力（Koleva等人，2012年）。