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Massively Parallel Reporter Assay for pluripotency factors in mESCs

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干细胞与再生医学数据中心2022-02-20 更新2024-03-06 收录
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A massively parallel reporter assay, MPRA, was conducted in mouse embryonic stem cells (mESC). Synthetic cis-regulatory elements comprised of binding sites for pluripotency transcription factors and genomic sequences with comparable binding sites configurations were used in the assay. Transcripts of dsRed were amplified via PCR from the end of the transcript to sequence 3' UTR barcodes.

提供机构:
Washington University
创建时间:
2022-02-20
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