Exogenous miRNA expression profiling in serum of pig and rat fed with different diets. Exogenous miRNA expression profiling in serum of pig and rat fed with different diets

We performed carefully designed and controlled animal feeding studies. We devided 9 adult rats in three group and each group fed with one type of diets in 28 days: rice, potatoes and chow. We fed piglets in triplicate either with cow milk or let them feed from sows milk for 4 weeks followed by 7 weeks of maize diet. Based on the small RNA sequencing data from 9 rat and 6 pig serum samples, we detected no transfer of plant miRNAs into rat blood, or bovine milk sequences into piglet blood. Overall design: The miRNA profiles in serum of chow-fed, potato-fed and rice-fed rats in 28 days and the miRNA profiles in serum of cow milk and sows milk fed pigs were generated by sequencing, in triplicate, Illumina TruSeq small RNA.