transcriptome of purified PC and non-PC fractions: GR-WT & GR-KO

Paneth Cells (PCs) are secretory cells located in the crypts of Lieberkuhn of the small intestine. They produce antimicrobial peptides (AMPs) and are thought to keep the microbiome under control. The cytokine TNF, which functions mainly via its TNF receptor 1 (TNFR1 or P55), is a driving force in many inflammatory bowel disease (IBD) patients, but its impact on PCs is poorly known. We have generated intestinal glucocorticoid receptor knockout mice (GR villin KO) and investigated the differences between WT and KO paneth and non-paneth cell fraction from the cell sorting. cells were isolated form naive mice that were either GRKO or GR WT. Both the PC and non-PC crypt cells were used for sequencing for a total of 4 conditions with 3 replicates each潘氏细胞（Paneth Cells，PCs）是定位于小肠李伯库恩隐窝的分泌型细胞，可产生抗菌肽（AMPs），被认为在维持肠道菌群稳态中发挥调控作用。肿瘤坏死因子（TNF）主要通过其肿瘤坏死因子受体1（TNF receptor 1，TNFR1或P55）发挥生物学功能，是多数炎症性肠病（IBD）患者发病的关键驱动因素，但其对潘氏细胞的影响目前尚不明确。本研究构建了villin启动子介导的肠道糖皮质激素受体敲除小鼠（GR villin KO），从未经过特殊处理的GR敲除（GRKO）与野生型（GR WT）小鼠中分离细胞，通过细胞分选获取潘氏细胞与非潘氏细胞组分，随后对两类隐窝细胞进行测序，共设置4组实验条件，每组设置3个生物学重复。