Transcriptome analysis of normal and cancerous bladder tissues.. Homo sapiens

The aim of this research was to explore activation/deactivation of signaling pathways during cancerogenesis. Overall design: Tissue samples from malignant tumors were obtained from patients who had undergone surgery. Tissue samples from non-cancer controls were collected from autopsies of healthy donors killed in road accidents. Both the tumors and normal tissues were evaluated by a pathologist to confirm the diagnosis and estimate the tumor cell numbers. All tumor samples used in this study contained at least 80% tumor cells. Tissue samples were immediately stabilized in RNAlater (Qiagen) and then stored at −80°C.