Single-Cell transcriptomes and immune profiling of IgG+ murine memory B cells from Bone Marrow and Spleen.

Single cell sequencing for analysis gene of gene transcription and Ig repertoires of isotype-switched IgG-expressing memory B cells for paired analysis with Ig repertoires in the same cells. Aim of the study was to explore heterogeneity of isotype-switched memory B cells within and between spleen and bone marrow compartments. Overall design: Sequencing of RNA and Ig was performed in duplicates of IgG+ B cells isolated from spleen and bone marrow of C57BL/6 mice.