Data_Sheet_3_Expansion and Divergence of Argonaute Genes in the Oomycete Genus Phytophthora.FASTA

Modulation of gene expression through RNA interference is well conserved in eukaryotes and is involved in many cellular processes. In the oomycete Phytophthora, research on the small RNA machinery and function has started to reveal potential roles in the pathogen, but much is still unknown. We examined Argonaute (AGO) homologs within oomycete genome sequences, especially among Phytophthora species, to gain a clearer understanding of the evolution of this well-conserved protein family. We identified AGO homologs across many representative oomycete and stramenopile species, and annotated representative homologs in P. sojae. Furthermore, we demonstrate variable transcript levels of all identified AGO homologs in comparison to previously identified Dicer-like (DCL) and RNA-dependent RNA polymerase (RDR) homologs. Our phylogenetic analysis further refines the relationship of the AGO homologs in oomycetes and identifies a conserved tandem duplication of AGO homologs in a subset of Phytophthora species.

通过RNA干扰调节基因表达在真核生物中得到了高度保守，且参与众多细胞过程。在卵菌门植物病原体疫霉菌中，对微小RNA机制及其功能的研究已开始揭示其在病原体中的潜在作用，然而仍有诸多未知。我们考察了卵菌门基因组序列中的Argonaute（AGO）同源基因，尤其在疫霉菌属中，以获得对该高度保守蛋白家族演化的更清晰认识。我们在众多代表性的卵菌门和藻类菌门物种中鉴定了AGO同源基因，并在P. sojae中注释了代表性同源基因。此外，我们比较了所有鉴定到的AGO同源基因的转录水平，与先前鉴定的Dicer样（DCL）和RNA依赖性RNA聚合酶（RDR）同源基因。我们的系统发育分析进一步细化了卵菌门中AGO同源基因之间的关系，并在疫霉菌属的某些物种中识别出保守的串联重复的AGO同源基因。