Regulation of fetoplacental metabolism in pig

RNA sequencing was performed to profile gene expression in the placentae of male and female fetusues (day 45) of pig. Genes showing sex-bias expression were identfieid. In this study, the Large White x Landrace gilts from the University of Missouri Swine Research Farm were used. The gilts were bred by artificial insemination (AI). The day of AI was considered as gestation day (GD) 1. On GD 45, pregnant pigs (n=6) were euthanized. The uterine horns were opened on the antimesometrial side and all the fetuses and corresponding placentae were removed. Total RNA from the male and female placentae (in triplicates) were used for RNA-seq analysis.