Eva Gluenz (2011) CIL:12404, Leishmania mexicana, parasite. CIL. Dataset

A murine macrophage phagocytosing a Leishmania parasite. Macrophages derived from the bone marrow of a C57/BL6 mouse were co-incubated in vitro with Leishmania mexicana (WHO strain MNYC/BZ/62/M379) stationary phase promastigotes for 30 min. The cells were then fixed with glutaraldehyde, dehydrated in ethanol, critical-point dried and sputter coated with gold. The image was captured on a JOEL JSM-6390 scanning electron microscope. Magnification 5000.

在此次实验中，我们观察到了小鼠巨噬细胞对利什曼原虫的吞噬过程。具体而言，源自C57/BL6小鼠骨髓的巨噬细胞，与处于静止期的利什曼原虫梅氏株（世界卫生组织菌株MNYC/BZ/62/M379）共培养30分钟。随后，使用戊二醛进行固定，以乙醇进行脱水处理，采用临界点干燥法干燥，并使用金溅射进行涂层处理。图像采集于JOEL JSM-6390扫描电子显微镜，放大倍数为5000倍。